Composite

Part:BBa_K3630025:Design

Designed by: Leong Chan Tat   Group: iGEM20_UM_Macau   (2020-10-26)


PCB formation LuxR-OmpC dependent expression system


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 7
    Illegal NheI site found at 30
    Illegal NheI site found at 1783
    Illegal NheI site found at 1806
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 4088
    Illegal XhoI site found at 2195
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal SapI.rc site found at 296


Design Notes

Ensure to remove all illegal restriction enzyme according to Biobrick compatibility rules


Source

Escherichia coli BL21

References